Effects of Allium sativum Bulb Extract, Diminazene Aceturate and Their Combination on Parasitaemia, and Biochemical Indices in Rats Experimentally Infected with Trypanosoma Brucei Brucei.

Thirty five clinically healthy albino rats of both sexes weighing between 100 – 120grams were used to study the effects of Allium sativum bulb extract in combination with diminazene aceturate on parsitemia and biochemical indices in trypanosome brucei brucei infection. The rats were divided in to seven groups (A-G) of five rats each. All the infected rats developed Parasitemia five days post infection. Weakness, increase respiratory rate, rough hair coat Biochemical changes at interval and possible deaths were the major parameters which were carefully observed. All the treatment commenced at the onset of parasitemia by day five post infection. Sub therapeutic dose of Allium sativum at 20mg/kg/bw in combination with 1.7mg/kg/bw of diminazene aceturate (Group C),diminazene aceturate at single standard dose of 3.5mg/kg/bw (Group B) caused a significant reduction (P<0.05) in parasitemia. The liver function enzymes ALT AST level in rats infected and not treated showed significant increase liver function enzymes (Group A) while those treated with standard and sub therapeutic dose (Group BCD) respectively. Had their liver function enzymes towards normal, compare with control (Group G).Its trypanocidal activity was assessed through daily examination of blood parasite, sub therapeutic doses of Allium sativum bulb extracts and its combination appear to be more effective in reducing severity of trypanosome brucei brucei infection and provide alternative in reducing the toxicity of existing trypanocide.

The modulatory effect of cochlospermum tinctorium a rich aqueous root extract on liver damage induced by carbon tetrachloride in rats

The aqueous root extract of Cochlospermum tinctorium (CTR) was investigated for its phytochemical composition; acute oral toxicity and hepatoprotective effect on carbon tetrachloride (CCl4) induced liver damage in rats. Phytochemical screening indicates the presence of alkaloids; tannins; cardiac glycosides; saponins; flavonoids; triterpenes; cyanogenic glycosides and volatile oils while steroids and anthraquinones were absent. Administration of 5000 mg/kg (body weight) of the extract orally did not produce any death in the rats within the observable period. The extract at 100 - 300 mg/kg (body weight) significantly and dose dependently reduced the levels of Alanine aminotransferase (ALT); Aspartate aminotransferase (AST) and Alkaline phosphatase (ALP) enzymes levels in the CCl4 -treated rats. The values of serum albumin; serum total protein and reduced glutathione in the extract treated groups of rats remained comparatively higher than its values in the CCl4 - treated group. The pretreatment of the rats with the extract produced a significant (P 0.05) reduction in blood clotting time. The histopathological findings were in support of the biochemical changes recorded during the study. These results suggest that aqueous root extract of CTR possess hepatoprotective effect against CCl4- induced liver damage in rats and the extract at 5000 mg/kg body weight appeared to be safe when administered orally